教育背景
1998-2002 北京大学生命科学学院 学士
2003-2009 美国加利福尼亚州大学戴维斯分校，博士
工作经历
2010-2011 美国加利福尼亚州大学旧金山分校 博士后
2011-2013 中国科学院生物物理研究所 副研究员
2013-2024 清华大学医学院基础医学系 副研究员
2024-至今 清华大学基础医学院 副研究员
研究领域
细胞遗传学和神经发育。结合超分辨活体成像、条件性基因编辑、基因组学分析等方法，研究细胞的分裂模式和细胞分化机制，神经前体细胞发育的调控机制。
科学贡献
发现调控不对称细胞分裂的微丝网络去分支蛋白、微管切割蛋白和细胞骨架极性扩张机制，以及表观遗传调控因子如何决定神经前体细胞命运的分子细胞学机制。阐明了膜骨架蛋白在时空维度上对细胞膜力学的调控，以及微丝细胞骨架如何调控凋亡细胞吞噬的机制。迄今作为第一作者或者通讯作者在Nature Biotechnology、Nature Protocols、PNAS、Journal of Cell Biology、Cell Discovery等国际期刊上发表多篇研究论文。
代表性论文
(1)Xie Z, Chai Y*, Zhu Z, Shen Z, Guo Z, Zhao Z, Xiao L, Du Z, Ou G, Li W*. Vacuolar H(+)-ATPase determines daughter cell fates through asymmetric segregation of the nucleosome remodeling and deacetylase complex. eLife. 2024; 12: RP89032.
(2)Shen Z, Guo Z, Ou G, Li W*. Inhibition of the chromatin remodeling factor NURF rescued sterility by a clinic variant of NuRD. Molecular Biology of the Cell, 2024,35(ar13): 1-9.
(3)Chai Y#, Tian D#, Zhu Z, Jiang Y, Huang S, Wu D, Ou G, Li W*. Wnt signaling polarizes cortical actin polymerization to increase daughter cell asymmetry. Cell Discovery. 2022, 8(1) .
(4)Xie C, Jiang Y, Huang S, Li W*, Ou G*. Actin Filament Debranching Regulates Cell Polarity during Cell Migration and Asymmetric Cell Division. Proceedings of the National Academy of the Sciences of the United States of America. 2021. 118(37). (*Correspondence author)
(5)Jia R, Chai Y, Xie C, Liu G, Zhu Z, Huang K, Li W*, Ou G*. The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans. J Cell Sci. 2020. 133(15). (*Correspondence author)
(6)Li W#, Li M, Ou G*.COVID‐19, cilia, and smell. The FEBS Journal. 2020, 287(17): 3672-3676.
(7)Wu D, Chai Y, Zhu Z, Li W, Ou G, Li W*. CED-10-WASP-Arp2/3 signaling axis regulates apoptotic cell corpse engulfment in C. elegans. Dev Biol. 2017;428(1):215-223
(8)Li W* and Ou G. The Application of Somatic CRISPR-Cas9 to Conditional Genome Editing in Caenorhabditis elegans. Genesis. 2016; 54(4): p. 170-81.
(9)Yi P, Li W*, Ou G*. The application of transcription activator-like effector nucleases for genome editing in C. elegans. Methods. 2014; 68(3):389-96.
(10)Cheng Z, Yi P, Wang X, Chai Y, Feng G, Yang Y, Liang X, Zhu Z, Li W*, Ou G*. Conditional targeted genome editing using somatically expressed TALENs in C. elegans. Nature Biotechnology. 2013; 31(10):934-7. (*Correspondence author)
(11)Li W#, Stuurman N. and Ou G. Chromophore-Assisted Laser Inactivation in Neural Development. Neuroscience Bulletin. 2012; 28(4):333-41.
(12)Chai Y#, Li W#, Feng G, Yang Y, Wang X and Ou G. Live Imaging of Cellular Dynamics during C. elegans Postembryonic Development. Nature Protocols. 2012; 7(12):2090-102.
(13)Li W#, Zou W†, Yang Y†, Chai Y†, Chen B, Cheng S, Tian D, Wang X, Vale RD and Ou G. Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell. Journal of Cell Biology. 2012; 197(1):27-35.
(14)Li W#, DeBella LR, Guven-Ozkan T, Lin R, and Rose LS. An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos. Journal of Cell Biology. 2009; 187:33-42.
