Education:
1995-2000 Medicine/BS Beijing University of Traditional Chinese Medicine
2003-2005 Chemistry/MS Utrecht University, Netherlands
2005-2010 Physics/PhD Leiden University, Netherlands
Professional Positions:
2010-2013 Post doctor, Institute of Chemistry, Chinese Academy of Sciences
2013-2024 Associate Researcher, Tsinghua University School of Medicine
2024-now Associate Researcher, Tsinghua University School of Basic Medical Sciences
Research Areas:
My scientific interest is on understanding the molecular basis of environmental perception of cell membrane and cross-membrane signal transduction. The research focuses on the interaction between lipid molecules and protein receptors on eukaryotic cell membranes. The classic theory of cell membrane receptor activation is based on ligand-receptor recognition, which centralizes protein-protein interactions only. However, an increasing amount of experimental data confirms that receptors can spontaneously undergo activation in the absence of ligands. This none ligand-dependent receptor activation typically occurs when the lipid microenvironment undergoes changes, indicating that lipid molecules play an important role in receptor activation. Therefore, the classic protein centered receptor activation theory needs to be improved and modified, and the interaction between proteins and lipid molecules should be included in the theory of receptor activation. With the help of advanced biophysical methods, on the one hand, the aggregation and movement status of receptors can be characterized in situ at the single-molecule level, and on the other hand, the purified transmembrane protein can be reconstructed in lipid micelle in vitro, and the influence of lipid composition on the structure of receptor transmembrane can be characterized by liquid nuclear magnetic resonance. Ultimately, we hope to gain a comprehensive understanding of the receptor activation on eukaryotic cell membranes.
Honors and Awards:
Ø 2011 China Postdoctoral Science Foundation Financial Support (No.201010490604)
Ø 2010 Chinese Academy of Sciences K. C. Wong Post-doctoral Fellowships
Selected Publications:
1.Li S, Huang F, Xia T, Shi Y, Yue T* (2023) Phosphatidylinositol 4,5-Bisphosphate Sensing Lipid Raft via Inter-Leaflet Coupling Regulated by Acyl Chain Length of Sphingomyelin, Langmuir 39: 5995-6005.
2.Yang C, Wang X, Xie R, Zhang Y, Xia T, Lu Y, Ye F, Zhang P, Cao T, Xu Y, Fan Q* (2023) Dynamically Reconstructed Collagen Fibers for Transmitting Mechanical Signals to Assist Macrophages Tracing Breast Cancer Cells, Advanced Functional Materials 33:9.
3.Xu Y, Song D, Wang W, Li S, Yue T, Xia T, Shi Y* (2023) Clec12a inhibits MSU-induced immune activation through lipid raft expulsion, Life Science Alliance 6:9.
4.Lyu H, Yuan G, Liu X, Wang X, Geng S, Xia T, Zhou X, Li Y, Hu X, Shi Y* (2023) Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs, eLife 12:257-277.
5.Wang X, Geng S, Meng J, Kang N, Liu X, Xu Y, Lyu H, Xu Y, Xu X, Song X, Zhang B, Wang X, Nuerbulati, Nuerdida, Zhang Z, Zhai D, Mao X, Sun R, Wang X, Wang R, Guo J, Chen, S R Wayne, Zhou X, Xia T, Qi, H, Hu X, Shi Y* (2023) Foxp3-mediated blockage of ryanodine receptor 2 underlies contact-based suppression by regulatory T cells, The Journal of clinical investigation 133:24.
6.Ruan H, Zou C, Xu Y, Fang X, Xia, T*, Shi Y* (2022) N-(3-Oxododecanoyl) Homoserine Lactone Is a Generalizable Plasma Membrane Lipid-Ordered Domain Modifier, FRONTIERS IN PHYSIOLOGY 12:257-277.
7.Xu S, Liu Y, Cernava, Tomislav, Wang H, Zhou Y, Xia T, Cao S, Berg, Gabriele, Shen X, Wen Z, Li C, Qu B, Ruan H, Chai, Y, Zhou X, Ma Z, Shi Y, Yu Y, Bai Y, Chen Y* (2022) Fusarium fruiting body microbiome member Pantoea agglomerans inhibits fungal pathogenesis by targeting lipid rafts, Nature Microbiology 7:831-843.
8.Liu L, Yu H, Zhao H, Wu Z, Long Y, Zhang J, Yan X, You Z, Zhou L, Du Y, Wang Y, Xia T, Shi Y, Xiao B, Huang C* (2020) Matrix-transmitted paratensile signaling enables myofibroblast-fibroblast cross talk in fibrosis expansion, Proceedings of the National Academy of Sciences of the United States of America 117:10832-10838.
9.Luo, F, Qin, G, Xia, T, Fang X* (2020) Single-Molecule Imaging of Protein Interactions and Dynamics, ANNUAL REVIEW OF ANALYTICAL CHEMISTRY 13:337-361.
10.Song D, Meng J, Ruan H, Kang N, Xu Y, Wang X, Shu F, Mu L, Li T, Ren W, Lin X, Xia T, Shi Y, Cheng J, Lyn J, Fan Z, Wu W, Chen P, Yan L, Tu Z, Li N, Fang X, Zhu J, Amrein, Matthias W.* (2019) Pseudomonas aeruginosa quorum-sensing metabolite induces host immune cell death through cell surface lipid domain dissolution, Nature Microbiology 4:97-111.
11.Chen J, Xu Y, Shi Y, Xia T* (2019) Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy, JOURNAL OF VISUALIZED EXPERIMENTS 2019:146
12.Zhao R, Yuan J, Li N, Sun Y, Xia T, Fang X* (2019) Analysis of the Diffusivity Change from Single-Molecule Trajectories on Living Cells, Analytical Chemistry 91:13390-13397.
13.Shu F, Chen J, Ma X, Fan Y, Yu L, Zheng W, Amrein M, Xia T, Shi Y* (2018) Cholesterol Crystal-Mediated Inflammation Is Driven by Plasma Membrane Destabilization, FRONTIERS IN IMMUNOLOGY 9:1163.
14.Mu L, Tu Z, Miao L, Ruan H, Kang N, Hei Y, Chen J, Wei W, Gong F, Wang B, Du Y, Ma G, Amerein, M, Xia T*, Shi Y* (2018) A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programing, NATURE COMMUNICATIONS 9:4259
15.Qi C, Li Y, Badger P, Yu H, You Z, Yan X, Liu W, Shi Y, Xia T, Dong J, Huang C, Du Y* (2017) Pathology-targeted cell delivery via injectable micro-scaffold capsule mediated by endogenous TGase, BIOMATERIALS 126:1-9.
16.Liu L, You Z, Yu H, Zhou L, Zhao H, Yan X, Li D, Wang B, Zhu L, Xu Y, Xia T, Shi Y, Huang C, Hou W, Du Y* (2017) Mechanotransduction-modulated fibrotic microniches reveal the contribution of angiogenesis in liver fibrosis, NATURE MATERIALS 16:1251-1261.
17.Amrein M, Xia T, Shi Y* (2017) Other modern methods for studying biomembranes, Membrane Biophysics: New Insights and Methods 393-421.
18.Chen J, Ganguly A, Mucsi A D, Meng J, Yan J, Detampel P, Munro F, Zhang Z, Wu M, Hari A, Stenner M D, Zheng W, Kubes P, Xia T, Amrein M, Qi H, Shi Y* (2017) Strong adhesion by regulatory T cells induces dendritic cell cytoskeletal polarization and contact-dependent lethargy, Journal of Experimental Medicine 214:327-338.
19.Wang B, Qin P, Zhao H, Xia T, Wang J, Liu L, Zhu L, Xu J, Huang C, Shi Y, Du Y* (2016) - Substrate stiffness orchestrates epithelial cellular heterogeneity with controlled proliferative pattern via E-cadherin/β-catenin mechanotransduction, Acta Biomaterialia 41:169-180.
20.Luo W, Xia T, Xu L, Chen Y, Fang X* (2014) Visualization of the post-Golgi vesicle-mediated transportation of TGF-beta receptor II by quasi-TIRFM, JOURNAL OF BIOPHOTONICS 7:788-798.
21.Xia T, Yuan J, Fang X* (2013) Conformational dynamics of an ATP-binding DNA aptamer: A single-molecule study, Journal of Physical Chemistry B 177:14994-15003.
22.Xia T, Li N, Fang X* (2013) Single-Molecule Fluorescence Imaging in Living Cells, ANNUAL REVIEW OF PHYSICAL CHEMISTRY 64:459-480.
23.Yuan H, Xia T, Plazanet M, Deme B, Orrit M* (2012) Communication: Crystallite nucleation in supercooled glycerol near the glass transition, JOURNAL OF CHEMICAL PHYSICS 136:041102
24.Yuan H, Xia T, Schuler B, Orrit M* (2011) Temperature-cycle single-molecule FRET microscopy on polyprolines, PHYSICAL CHEMISTRY CHEMICAL PHYSICS: 13:1762-1769.
25.Kulzer F, Xia T, Orrit M* (2010) Single Molecules as Optical Nanoprobes for Soft and Complex Matter, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 49:854-866.
26.Möbius M, Xia T, van Saarloos W, Orrit M, van Hecke M* (2010) Aging and Solidification of Supercooled Glycerol, JOURNAL OF PHYSICAL CHEMISTRY B 114:7439-7444.
27.Xia T, Xiao L, Orrit M* (2009) Micron-Sized Structure in a Thin Glycerol Film Revealed by Fluorescent Probes, JOURNAL OF PHYSICAL CHEMISTRY B 113:15724-15729.
28.Zondervan R, Xia T, van der Meer H, Storm C, Kulzer F, van Saarloos W, Orrit M* (2008) Soft glassy rheology of supercooled molecular liquids, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105:4993-4998.
