Education
1989, Ph.D, Animal genetics and breeding, North West Agriculture and Forestry University, Shaanxi, Yangling, China;
1987, B.S. Animal genetics and breeding, North West Agriculture and Forestry University, Shaanxi, Yangling, China.
Appointments
2012 ~ Principal Investigator, Tenured Professor, School of Medicine, School of Life Sciences, Tsinghua University
2006 ~ 2012 Principal Investigator, Professor, School of Medicine, School of Life Sciences, Tsinghua University
1998 ~ 2005 Principal Investigator, Associate professor, School of Medicine, School of Life Sciences, Tsinghua University
2006 ~ 2006: Visiting Scientist. Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
2000 ~ 2000: Visiting Scientist. Forsyth Research Institute, Harvard School of Dental Medicine, Boston, MA.
2000 ~ 2000: Visiting Scientist. University of Toronto, Toronto, Canada.
1997 ~ 1998: Postdoctoral research. Department of Pathology, University of Alabama at Birmingham, AL 35294, USA.
1996 ~ 1997: Exchange scholar training, Renal Division/Jewish and Barnes Hospital, Washington University Medical Center, St. Louis, MO 63110, USA
1995 ~ 1996 : Exchange scholar training, Department of Pharmacological, Physio-logical Sciences, St. Louis University Medical School, St. Louis, MO 63214, USA.
1989 ~ 1995: Associate Professor. Department of Animal Science, The Northwestern Agricultural University, Shaanxi, China.
Research Fields, Emphasis
Dr. Zhijie Chang's current research focuses on the mechanism of cell signal transduction and tumorigenesis, with particular attention to the role of key proteins in signal pathways such as STAT3, WNT, and NF-kB in regulating gene expression during tumorigenesis. Using molecular, cellular and animal models, he works to explore the function and mechanism of oncogene CREPT in various tumors, and develop new anti-tumor inhibitors and biomarkers for clinical diagnosis. He also works on the mechanisms of chronic inflammation on diseases and develops tools including mesenchymal stem cells and exosomes for the therapy of inflammation related diseases such as fibrosis and wound healing.
Academic Awards
2003 First Merit Paper Award.Awarded by Chinese Cell Biology Society.
2005 Third Merit Paper Award. Awarded by Chinese Cell Biology Society.
2007 Third Merit Paper Award. Awarded by Chinese Cell Biology Society.
2007 Third Merit Paper Award. Awarded by Chinese Cell Biology Society.
2009 Third Merit Paper Award. Awarded by Chinese Cell Biology Society.
2010 Bayer-Tsinghua Investigator Award.
2013 Bayer-Tsinghua Investigator Award.
2015 Bayer-Tsinghua Investigator Award.
Selected Publications
1. Mengdi Li, Yuting Lin, Jiayu Wang, He Yang, Danhui Ma, Ye Tian, Yi Wang, Liu Yang, Umar Farooq, Yinyin Wang, Fangli Ren, Jian Sheng, Guoqing Zhang, Liang Chen, Jun Li, Xiangnan Li*, Zhijie Chang*, CREPT promotes LUAD progression by enhancing the CDK9 and RNAPII assembly to promote ERK-driven gene transcription. Theranostics. 2025 Jul 25;15(16):8337-8359. doi: 10.7150/thno.115572. PMID: 40860160; PMCID: PMC12374588.
2. Umar Farooq, Jun Li, Zhijie Chang*, Disrupting Cell Cycle Machinery: CREPT Is an Emerging Target in Cancer Therapy, Cancers (Basel). 2025 Jul 19;17(14):2401. doi: 10.3390/cancers17142401.
3. Tingwei Lan, Mengdi Li, Xiaolin Duan, Huihui Jia, Yajun Cao, Yinyin Wang, Fangli Ren, Jianqiu Sheng, Junfeng Xu*, Zhijie Chang*, Regulation of revival stem cell differentiation by CREPT/RPRD1B during intestinal regeneration, Cell, Bioscience, 2025 15:98, https://doi.org/10.1186/s13578-025-01434-6.
4. Jianghua Li, Lu Xu, Jiayu Wang, Xuning Wang, Yuting Lin, Alex Yutian Zou, Fangli Ren, Yinyin Wang*, Jun Li*, Zhijie Chang*, CREPT is required for the metastasis of triple-negative breast cancer through a co-operational-chromatin loop-based gene regulation, Molecular Cancer, 2025 24:170, https://doi.org/10.1186/s12943-025-02361-3.
5. Meng Li , Huaping Huang , Xiaofei Wei, Huajuan Li, Jun Li, Bingchen Xie, Yuze Yang, Xingyue Fang, Lei Wang, Xiaona Zhang, Heyu Wang, Mengdi Li, Yuting Lin, Dezhi Wang, Yinyin Wang, Tongbiao Zhao, Jianqiu Sheng, Xinbao Hao, Muyang Yan*, Lu Xu*, Zhijie Chang*, Clinical investigation on nebulized human umbilical cord MSC-derived extracellular vesicles for pulmonary fibrosis treatment, Signal Transduct Target Ther. 2025 Jun 4;10(1):179. doi: 10.1038/s41392-025-02262-3.
6. Mengdi Li, Jingya Li, Chunhua He, Guancheng Jiang, Danhui Ma, Haipeng Guan, Yuting Lin, Meng Li, Jing Jia, Xiaolin Duan, Yinyin Wang, Fangli Ren, Haitao Li, Xiaoguang Wang, Chenxi Cao*, Zhijie Chang*, An oncoprotein CREPT functions as a co-factor in MYC-driven transformation and tumor growth, J Biol Chem. 2025 Jan;301(1):108030. doi: 10.1016/j.jbc.2024.108030.
7. Meng Li, Jun Li, Ying Wang, Guancheng Jiang, Hanguo Jiang, Mengdi Li, Ziying Zhu, Fangli Ren, Yinyin Wang, Muyang Yan, Zhijie Chang*, Umbilical cord-derived mesenchymal stem cells preferentially modulate macrophages to alleviate pulmonary fibrosis, Stem Cell Res Ther. 2024 Dec 18;15(1):475. doi: 10.1186/s13287-024-04091-7.
8. Yuting Lin, Hanguo Jiang, Jun Li, Fangli Ren, Yinyin Wang, Ying Qiu, Jianghua Li, Mengdi Li, Ying Wang, Liu Yang, Yunhao Song, Huihui Jia, Wanli Zhai, Yanshen Kuang, Hanyang Yu, Wenyuan Zhu, Suling Liu, Eiichi Morii, Christian Ensinger, Charles David, Hanqiu Zheng, Jianguo Ji, Hongxia Wang*, Zhijie Chang*, Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization, Theranostics, 2024; 14(2): 662-680. doi: 10.7150/thno.87344
9. Jun Chu, Wanli Zhai, Yongtao Geng, Yarui Feng, Jiayu Wang, Jun Li, Yinyin Wang, Wenying Zhuang, Yongsheng Che, Yi Li, Zhijie Chang*, Fangli Ren*. Lachnochromonin, a fungal metabolite from Lachnum virgineum, inhibits cell growth and promotes apoptosis in tumor cells through JAK/STAT3 signaling. Cellular Signalling. Volume 106, June 2023, 110592.
10. Guochun Zhu, Wei Chen, Chen-Yi Tang, Abigail McVicar, Diep Edwards, Jinwen Wang, Matthew McConnell, Shuying Yang, Yang Li, Zhijie Chang*, Yi-Ping Li*. Knockout and Double Knockout of Cathepsin K and Mmp9 reveals a novel function of Cathepsin K as a regulator of osteoclast gene expression and bone homeostasis. Int J Biol Sci. 2022 Aug 29;18(14):5522-5538. doi: 10.7150/ijbs.72211. eCollection 2022.
11. Yajun Cao, Bobin Ning, Ye Tian, Tingwei Lan, Yunxiang Chu, Fangli Ren, Yinyin Wang, Qingyu Meng, Jun Li, Baoqing Jia*, Zhijie Chang*. CREPT disarms the inhibitory activity of HDAC1 on oncogene expression to promote tumorigenesis. Cancers. 2022, 14, 4797. Doi: 10.3390/cancers14194797.
12. Yanxia Fu, Jun Li, Ziyu Zhang, Fangli Ren, Yinyin Wang, Huihui Jia, Jehe Liu, Zhijie Chang*. Umbilical cord mesenchymal stem cell-derived exosomes alleviate collagen-induced arthritis by balancing the population of Th17 and regulatory T cells. FEBS Letters. 2022. Accepted.
13. Yanxia Fu, Jun Li, Mengdi Li, Junfeng Xu, Zheng Rong, Fangli Ren, Yinyin Wang, Jianqiu Sheng, Zhijie Chang*. Umbilical cord mesenchymal stem cells ameliorate inflammation-related tumorigenesis via modulating macrophages. Stem Cells International. 2022. doi: 10.1155/2022/1617229.
14. Sida Guo, Jun Fang, Weizhi Xu, Janice Ortega, Chang-Yi Liu, Liya Gu, Zhijie Chang*, and Guo-Min Li*. Interplay between H3K36me3, methyltransferase SETD2, and mismatch recognition protein MutSα facilitates processing of oxidative DNA damage in human cells. J. Biol. Chem. (2022) in press.
15. Yanxia Fu, Sihan Liu, Mengdi Li, Fangli Ren, Yinyin Wang, Zhijie Chang*. IL-17RD/sef exacerbates experimental mouse colitis and inflammation-associated tumorigenesis by regulating the proportion of T cell subsets. FEBS Letters. 2022. Feb;596(4):427-436.doi: 10.1002/1873-3468.14266. Epub 2022 Jan 21.
16. Qiong Wu, Yaping Huang, Liya Gu, Zhijie Chang*, and Guo-Min Li*. OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. J. Biol. Chem. (2021) 296:100466. DOI: 10.1016/j.jbc.2021.100466.
17. Wanli Zhai, Xiongjun Ye, Yinyin Wang, Yarui Feng, Ying Wang, Yuting Lin, Lidan Ding, Liu Yang, Xuning Wang, Yanshen Kuang, Xinyuan Fu, Y. Eugene Chin, Baoqing Jia, Bingtao Zhu*, Fangli Ren*, Zhijie Chang*. CREPT/RPRD1B promotes tumorigenesis through STAT3-driven gene transcription in a p300-dependent manner. British Journal of Cancer. (2021) 124:1437–1448; https://doi.org/10.1038/s41416-021-01269-1.
18. Liu Yang, Haiyan Yang, Yunxiang Chu, Yunhao Song, Lidan Ding, Bingtao Zhu, Wanli Zhai, Xuning Wang, Yanshen Kuang, Fangli Ren, Baoqing Jia, Wei Wu, Xiongjun Ye*, Yinyin Wang* and Zhijie Chang*. CREPT is required for murine stem cell maintenance during intestinal regeneration. Nature Communications, (2021) 12:270 doi:10.1038/s41467-020-20636.
19. Mengdi Li, Danhui Ma, Zhijie Chang*. Current understanding of CREPT and p15RS, carboxy-terminal domain (CTD)-interacting proteins, in human cancers. Oncogene. 40:705–716 (2021), Epub: 2020 Nov 25. doi: 10.1038/s41388-020-01544-0.
20. Yajun Cao, Hongyan Wu, Wanli Zhai, Ying Wang, Mengdi Li, Meng Li, Liu Yang, Ye Tian, Yunhao Song, Jun Li, Yinyin Wang, Qiang Din, Lingqi Zhang, Ming Cai*, Zhijie Chang*. A safety consideration of mesenchymal stem cell therapy on COVID-19. Stem Cell Research. 49:102066, doi: 10.1016/j.scr.2020.102066. Epub 2020 Oct 24, 2020.
21. Yinyin Wang, Sihan Liu, Guancheng Jiang, Wanli Zhai, Liu Yang, Mengdi Li, Zhijie Chang*, Bingtao Zhu*, NOK associates with c-Src and promotes c-Src-induced STAT3 activation and cell proliferation. Cell Signal. 2020 Aug 29;75:109762. doi: 10.1016/j.cellsig.2020.109762.
22. Sihan Liu, Yanxia Fu, Kunrong Mei, Yinan Jiang, Xiaojun Sun, Yinyin Wang, Fangli Ren, Congshan Jiang, Liesu Meng, Shemin Lu, Zhihai Qin, Chen Dong, Xinquan Wang, Zhijie Chang* and Shigao Yang*. A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering. Cellular, Molecular Immunology. 2020 Sep 22. doi: 10.1038/s41423-020-00548-w.
23. Danhui Ma, Yutian Zou, Yunxiang Chu, Zhengsheng Liu, Gaochao Liu, Jun Chu, Mengdi Li, Jiayu Wang, Shi-yong Sun*, Zhijie Chang*. A cell-permeable peptide-based PROTAC against the oncoprotein CREPT proficiently inhibits pancreatic cancers. Theranostics. 2020, 10(8): 3708-3721. doi: 10.7150/thno.41677.
24. Fu Y, Kong Y, Li J, Wang Y, Li M, Wang Y, Wang Y, Ren F, Ni J, Li Y, Chang Z*. Mesenchymal stem cells combined with traditional Chinese medicine (qi-fang-bi-min-tang) alleviates rodent allergic rhinitis. J Cell Biochem. 2020 Feb; 121(2):1541-1551. doi: 10.1002/jcb.29389. Epub 2019 Sep 18.
25. Liu H, Seynhaeve ALB, Brouwer RWW, van IJcken WFJ, Yang L, Wang Y, Chang Z*, Ten Hagen TLM*. CREPT Promotes Melanoma Progression Through Accelerated Proliferation and Enhanced Migration by RhoA-Mediated Actin Filaments and Focal Adhesion Formation. Cancers (Basel). 2019 Dec 20; 12(1). pii: E33. doi: 10.3390/cancers12010033.
26. Yu S#, Huang H#, Wang S#, Xu H, Xue Y, Huang Y, He J, Xu X, Wu Z, Wu J, Zhang Y, Huang Q, Chang Z*, Li E*, Xu L*. CREPT is a novel predictor of the response to adjuvant therapy or concurrent chemoradiotherapy in esophageal squamous cell carcinoma. Int J Clin Exp Pathol. 2019 Sep 1;12(9):3301-3310. eCollection 2019. PubMed PMID: 31934173; PubMed Central PMCID: PMC6949861
27. Yanxia Fu, Sihan Liu, Yinyin Wang, Fangli Ren, Xuanzi Fan, Jiao Liang, Chunxiao Liu, Jun Li, Yanfang Ju, Zhijie Chang*. GdX/UBL4A knockout mice resist collagen-induced arthritis by balancing the population of Th1/Th17 and regulatory T cells. The FASEB Journal. 2019 Jul; 33(7):8375-8385. doi: 10.1096/fj.201802217RR. Epub 2019 Apr 19.
28. Chunxiao Liu, Yifan Zhou, Mengdi Li, Ying Wang, Liu Yang, Shigao Yang, Yarui Feng, Yinyin Wang, Yangmeng Wang, Fangli Ren, Jun Li, Zhongjun Dong, Y. Eugene Chin, Xinyuan Fu, Li Wu*, Zhijie Chang*. Absence of GdX/UBL4A protects against inflammatory diseases by regulating NF-kB signaling in macrophages and dendritic cells. Theranostics. 2019; 9(5): 1369-1384. doi: 10.7150/thno.32451.
29. Lidan Ding, Liu Yang, Yuqi He, Bingtao Zhu, Yinyin Wang, Mengdi Li, Fangli Ren, Jun Li, Sihan Liu, Yanshen Kuang, Wanli Zhai, Danhui Ma, Xuanzi Fan, Yanfang Ju, Quentin Liu, Baoqing Jia, Jianqiu Sheng*, Zhijie Chang*. CREPT associates with Aurora B to regulate cyclin B1 expression for accelerating G2/M transition in gastric cancer. Cell Death and Disease. 2018 Dec 5; 9(12):1172. doi: 10.1038/s41419-018-1211-8.
30. Jian Li, Amber R. Smith, Rebecca T. Marquez, Jun Li, Kun Li, Lan Lan, Xiaoqing Wu, Linxi Zhao, Fangli Ren, Yi Wang, Yinyin Wang, Baoqing Jia, Liang Xu* and Zhijie Chang*. MicroRNA-383 acts as a tumor suppressor in colorectal cancer by modulating CREPT/RPRD1B expression. Molecular Carcinogenesis. 2018 Oct;57(10):1408-1420. doi: 10.1002/mc.22866. Epub 2018 Jul 3.
31. Xuanzi Fan#, Juan Zhao#, Fangli Ren, Yinyin Wang, Yarui Feng, Lidan Ding, Linpeng Zhao, Jun Li, Jianquan Ni, Baoqing Jia, Zhijie Chang*. Dimerization of p15RS mediated by a leucine-zipper-like motif is critical for its inhibitory role on Wnt signaling. J. Biol. Chem. 2018 May 18;293(20):7618-7628. doi: 10.1074/ jbc. RA118.001969. Epub 2018 Apr 4.
32. Yanquan Zhang, Shiyan Wang, Wei Kang, Chunxiao Liu, Yujuan Dong, Fangli Ren, Yinyin Wang, Jinglin Zhang, Guoping Wang, Ka-Fai To, Xueji Zhang, Joseph Sung, and Zhijie Chang*, Jun Yu*. CREPT facilitates colorectal cancer growth through inducing Wnt/b-catenin pathway by enhancing p300-mediated b-catenin acetylation. Oncogene, 2018 Mar 22. doi: 10.1038/s41388-018-0161-z. [Epub ahead of print]. (co-corresponding authors).
33. Jiao Liang, Ying Qiu, Yinyin Wang, Fangli Ren, Zhijie Chang*. CREPT expression correlates with esophageal squamous cell carcinoma histological grade and clinical outcome. Int J Clin Exp Pathol 2017;10(2):2030-2035.
34. Yu Shang, Jinjun He, Yinyin Wang, Quanling Feng, Yanquan Zhang, Junwei Guo, Jun Li, Shan Li, Yangmeng Wang, Ge Yan, Fangli Ren, Yuguang Shi, Jiake Xu, Nikolajs Zeps, Yonggong Zhai, Dacheng He, and Zhijie Chang*. CHIP/Stub1 Regulates the Warburg Effect by Promoting Degradation of PKM2 in Ovarian Carcinoma. Oncogene. 36(29):4191-4200, Jul 20, 2017. doi: 10.1038/onc.2017.31. Epub 2017 Mar 27.
35. Li Ma, Chao Huang*, Dazhuan Eric Xin, Xiong-Jun Wang, Li-shun Wang, Quanli C. Zou, Ya-nan S. Zhang, Min-dian Tan, Yu-mei Wang, Ting C. Zhao, Devasis Chatterjee, Rachel A. Altura, Chuangui Wang, Yan S. Xu, Jing-hua Yang, Yong-sheng Fan, Bao-hui Han, Jianmin Si, Jinke Cheng, Zhijie Chang*, Y. Eugene Chin*. Lysyl Oxidase 3 Is a Dual-Specificity Enzyme Involved in STAT3 Deacetylation and Deacetylimination Modulation. Mol Cell, 65(2), 296–309, 2017 (co-corresponding authors). doi: 10.1016/j.molcel.2016.12.002. Epub 2017 Jan 5.
36. Fangli Ren, Yongtao Geng, Takayuki Minami, Ying Qiu, Yarui Feng, Chunxiao Liu, Juan Zhao, Yinyin Wang, Xuanzi Fan, Yangmeng Wang, Mengdi Li, Jun Li*, and Zhijie Chang*. Nuclear termination of STAT3 signaling through the SIPAR (STAT3-Interacting Protein As a Repressor) adaptor recruiting T cell tyrosine phosphatase TC-PTP. FEBS Letters. Jul 8; 589(15):1890-6 2015. doi: 10.1016/j.febslet.2015.05.031. Epub 2015 May 27.
37. Chunxiao Liu, Yanquan Zhang, Jun Li, Yinyin Wang, Fangli Ren, Yifan Zhou, Yinyuan Wu, Yarui Feng, Yu Zhou, Fuqin Su, Baoqing Jia, Dong Wang, and Zhijie Chang*. p15RS(p15INK4b Related Sequence)/RPRD1A (Regulation of Nuclear Pre-mRNA Domain-Containing Protein 1A) Interacts with HDAC2 (Histone Deacetylase 2) in Inhibition of the Wnt/b-catenin Signaling Pathway. J. Biol. Chem. 2015 Apr 10;290(15):9701-13. doi: 10.1074/jbc.M114.620872. Epub 2015 Feb 19.
38. Shigao Yang, Yinyin Wang, Kunrong Mei, Sen Zhang, Xiaojun Sun, Fangli Ren, Sihan Liu, Zi Yang, Xinquan Wang, Zhihai Qin, Zhijie Chang*. TNFR2–IL-17RD heteromerization reveals a novel mechanism for NF-κB activation. J. Biol. Chem. 2015 Jan 9; 290(2):861-71. doi: 10.1074/jbc.M114.586560.
39. Fangli Ren, Ruoke Wang, Yanquan Zhang, Chunxiao Liu, Yinyin Wang, Jim Hu, Linqi Zhang, and Zhijie Chang*. Characterization of a Monoclonal Antibody Against CREPT, a Novel Protein Highly Expressed in Tumors. Monoclon Antib Immunodiagn Immunother. 33(6): 401–408, 2014. doi: 10.1089/mab.2014.0043.
40. Yanquan Zhang, Chunxiao Liu, Xiaolin Duan, Fangli Ren, Zhe Jin, Shan Li, Yinyin Wang, Yarui Feng, Zewen Liu and Zhijie Chang*. CREPT/RPRD1B, a Recently Identified Novel Protein Highly Expressed in Tumors, Enhances the b-catenin/TCF4 Transcriptional Activity in Response to Wnt Signaling. J. Biol. Chem. 289(33):22589-99, 2014. doi: 10.1074/jbc.M114.560979.
41. Yongtao Geng, Yanfang Ju, Fangli Ren, Ying Qiu, Yasuhiko Tomita, Miki Tomoeda, Mioka Kishida, Yinyin Wang, Lian Jin, Fuqin Su, Chunhong Wei, Baoqing Jia, Yi Li*, Zhijie Chang*. Insulin Receptor Substrate 1/2 (IRS1/2) Regulates Wnt/β-catenin Signaling through Blocking Autophagic Degradation of Dishevelled2. J. Biol. Chem. Apr 18; 289(16): 11230-41, 2014. (co-corresponding authors) doi: 10.1074/jbc.M113.544999
42. Huabing Qi, Min Jin, Yaqi Duan, Xiaolan Du, Yuanquan Zhang, Fangli Ren, Yinyin Wang, Qingyun Tian, Xiaofeng Wang, Quan Wang, Ying Zhu, Yangli Xie, Chuanju Liu, Xu Cao, Yuji Mishina, Di Chen, Chu-xia Deng, Zhijie Chang*, Lin Chen*. FGFR3 induces degradation of BMP type I receptor to regulate skeletal development. BBA-Molecular Cell Research. Jul; 1843(7): 1237-47, 2014. doi: 10.1016/j.bbamcr.2014.03.011 (co-corresponding authors).
43. Yu Shang, Xialian Xu, Xiaolin Duan, Junwei Guo, Yinyin Wang, Fangli Ren, Dacheng He and Zhijie Chang*. Hsp70 and Hsp90 oppositely regulate TGF-b signaling through CHIP/Stub1. Bioch. Bioph. Res. Com. 421:651-657, 2014. doi: 10.1016/j.bbrc.2012.04.049
44. Shan Li, Bing Shu, Yanquan Zhang, Jia Li, Junwei Guo, Yinyin Wang, Fangli Ren, Guozhi Xiao, Zhijie Chang*, and Di Chen*. CHIP Regulates Osteoclast Formation through Promoting TRAF6 Protein Degradation. Arthritis, Rheumatology. 66(7):1854-1863, 2014. doi: 10.1002/art.38521 (co-corresponding authors).
45. Yu Shang, Xinghui Zhao, Bo Tian, Yinyin Wang, Fangli Ren, Baoqing Jia, Yonggong Zhai, Dacheng He, Wei Chen and Zhijie Chang*. CHIP/Stub1 interacts with eIF5A and mediates its degradation. Cellular Signalling. 26: 1098-1104, 2014. doi: 10.1016/j.cellsig.2014.01.030.
46. Yangmeng Wang, Hongxiu Ning, Fangli Ren, Yuanjiang Zhang, Yu Rong, Yinyin Wang, Fuqin Su, Chenguang Cai, Zhe Jin, Zhiyong Li, Xinqi Gong, Yonggong Zhai, Dianjun Wang, Baoqing Jia, Ying Qiu, Yasuhiko Tomita, Joseph JY Sung, Jun Yu, David M. Irwin, Xiao Yang, Xinyuan Fu, Y. Eugene Chin*, and Zhijie Chang*. GdX/UBL4A Specifically Stabilizes the TC45/STAT3 Association and Promotes Dephosphorylation of STAT3 to Repress Tumorigenesis. Mol Cell. 53:752-765, 2014. doi: 10.1016/j.molcel.2014.01.020.
47. Yangmeng Wang, Fangli Ren, Yinyin Wang, Yarui Feng, Dianjun Wang, Baoqing Jia, Ying Qiu, Shiyan Wang, Jun Yu, Joseph JY Sung, Jiake Xu, Nikolajs Zeps, and Zhijie Chang*. CHIP/Stub1 functions as a tumor suppressor and represses NF-κB-mediated signaling in colorectal cancer. Carcinogenesis. May;35(5):983-91, 2014. doi: 10.1093/carcin/bgt393.
48. Mei KunRong, Jin Zhe, Ren Fangli, Wang Yinyin, Chang Zhijie*, Wang Xinquan*. Structure basis for the recognition of RNA polymerase II C-terminal domain by CREPT and p15RS. Science China Life Sciences. Volume 57, Number 1:97-106, 2014. doi: 10.1007/s11427-013-4589-7 (co-corresponding authors).
49. Hongge Li, Songchang Guo, Yongming Ren, Depeng Wang, Honghao Yu, Wenjing Li, Xinquan Zhao, and Zhijie Chang*. VEGF189 Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes. HIGH ALTITUDE MEDICINE, BIOLOGY. Volume 14, 2013 Dec;14(4):395-404. doi: 10.1089/ham.2013.1013.
50. Fangli Ren, Fuqin Su, Hongxiu Ning, Yangmeng Wang, Yongtao Geng, Yarui Feng, Yinyin Wang, Yanquan Zhang, Zhe Jin, Yi Li, Baoqing Jia and Zhijie Chang*. SIPAR negatively regulates STAT3 signaling and inhibits progression of melanoma. Cellular Signalling. 25:2272-2280, 2013. doi: 10.1016/j.cellsig. .2013.07.023.
51. Xiaojun Sun, Yinyin Wang, Shigao Yang, Fangli Ren, Yongjing Xia, Zhijie Chang*. Activation of TAK1 by Sef-S induces apoptosis in 293T cells. Cellular Signalling. 25:2039-2046, 2013. doi: 10.1016/j.cellsig.2013.06.006.
52. Xi Yang, Yanquan Zhang, Shan Li, Chunxiao Liu, Zhe Jin, Yinyin Wang, Fangli Ren, Zhijie Chang*. Rab21 attenuates EGF-mediated MAPK signaling through enhancing EGFR internalization and degradation. Bioch. Bioph. Res. Com. 421:651-657, 2012. doi: 10.1016/j.bbrc.2012.04.049.
53. Junwei Guo, Fangli Ren, Yinyin Wang, Shan Li, Zhengrong Gao, Xiaoyan Wang, Hongxiu Ning, Jianguo Wu, Yi Li, Zhao Wang, Shek Man Chim, Jiake Xu, and Zhijie Chang*. miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression. Journal of Bone and Mineral Research. 2012 Mar 9. doi: 10.1002/jbmr.1597. [Epub ahead of print]
54. Fuqin Su, Fangli Ren, Yu Rong, Yangmeng Wang, Yongtao Geng, Yinyin Wang, Mengyao Feng, Yanfang, Ju Yi Li, Zhizhuang J. Zhao, Kun Meng*, Zhijie Chang*. Protein tyrosine phosphatase Meg2 dephosphorylates STAT3 and suppresses tumor growth in the breast cancer. Breast Cancer Research. 2012 Mar 6;14(2):R38. doi：10.1186/bcr3134.
55. Dongdong Lu, Yinyuan Wu, Yinyin Wang, Fangli Ren, Dianjun Wang, Fuqin Su, Yanquan Zhang, Xi Yang, Guihua Jin, Xinbao Hao, Dacheng He, Yonggong Zhai, David M Irwin, Jim Hu, Joseph JY Sung, Jun Yu, Baoqing Jia*, Zhijie Chang*. CREPT accelerates tumorigenesis by regulating the transcription of cell-cycle related genes. Cancer Cell. 21:92-104, 2012. doi: 10.1016/j.ccr.2011.12.016.
56. Yangmeng Wang, Dianjun Wang, Fangli Ren, Yuanjiang Zhang, Fuyu Lin, Ning Hou, Xuan Cheng, Peng Zhang, Yinyin Wang, Baoqing Jia, Xiao Yang and Zhijie Chang*. Generation of mice with conditional null allele for GdX/Ubl4A. Genesis. 50:534-542, 2012. doi: 10.1002/dvg.20832.
57. Yanquan Zhang, Fang Wang, Liang Han, Yinyuan Wu, Shan Li, Xi Yang, Yinyin Wang, Fangli Ren, Yonggong Zhai, Dianjun Wang, Baoqing Jia, Yongjing Xia, and Zhijie Chang*. GABARAPL1 negatively regulates Wnt/b-catenin pathway by mediating Dvl2 degradation through autophagy pathway. Cellular Physiology and Biochemistry. 27:503-512, 2011. doi: 10.1159/000329952.
58. Xiaoyan Wang, Kenichi Harimoto, Jing Liu, Junwei Guo, Stephen Hinshaw, Zhijie Chang*, Zhao Wang*. Spata4 promotes osteoblast differentiation through Erk-activated Runx2 pathway. Journal of Bone and Mineral Research 26(8):1964-73, 2011. doi: 10.1002/jbmr.394. (co-corresponding authors).
59. Yinyuan Wu, Dianjun Wang, Yinyin Wang, Fangli Ren, Donald Chang, Zhijie Chang* and Baoqing Jia*. Caspase 3 is activated through caspase 8 instead of caspase 9 during H2O2-induced apoptosis in HeLa cells. Cellular Physiology and Biochemistry. 27:539-546, 2011. doi: 10.1159/000329955. (co-corresponding authors).
60. Xinbao Hao, Yinyin Wang, Fangli Ren, Shanshan Zhu, Yongming Ren, Baoqing Jia, Yi-Ping Li, Yuguang Shi and Zhijie Chang*. SNX25 regulates TGF-β signaling by enhancing the receptor degradation. Cellular Signalling. 23(5):935-946, 2011. doi: 10.1016/j.cellsig.2011.01.022.
61. Yinyuan Wu, Yanquan Zhang, Haiwei Zhang, Xi Yang, Yinyin Wang, Fangli Ren, Huitu Liu, Yonggong Zhai, Baoqing Jia, Jun Yu, and Zhijie Chang*. p15RS attenuates Wnt/β-catenin signaling by disrupting β-catenin/TCF4 interaction. J. Biol. Chem. 285(45):34621-34631, 2010. doi: 10.1074/jbc.M110.148791.
62. Haiwei Zhang, Hui Zhang, Yanquan Zhang, Ser Sue Ng, Fangli Ren, Yingying Wang, Yaqi Duan, Lin Chen, Yonggong Zhai, Qinglong Guo*, and Zhijie Chang*. Dishevelled-DEP domain interacting protein (DDIP) inhibits Wnt signaling by promoting TCF4 degradation and disrupting the TCF4/b-catenin complex. Cellular Signalling.22:1753-1760, 2010. doi: 10.1016/j.cellsig.2010.06.016. (co-corresponding authors).
63. Yinyuan Wu, Yinyin Wang, Yang Sun, Liying Zhang, Dianjun Wang, Fangli Ren, Donald Chang, Zhijie Chang* and Baoqing Jia*. RACK1 promotes Bax oligomerization and dissociates the interaction of Bax and Bcl-XL. Cellular Signalling. 2010; 22:1495-1501. doi: 10.1016/j.cellsig.2010.05.018. (co-corresponding authors).
64. Yu Shang, Xinghui Zhao, Xialian Xu, Hong Xin, Xueni Li, Yonggong Zhai, Dacheng He, Baoqing Jia, Wei Chen, and Zhijie Chang*. CHIP functions as an E3 ubiquitin ligase of Runx1. Bioch. Bioph. Res. Com. 386:242-246, 2009. doi: 10.1016/j.bbrc.2009.06.043.
65. Zhen Qin, Fangli Ren, Xialian Xu, Yongming Ren, Hongge Li, Yinyin Wang, Yonggong Zhai, and Zhijie Chang*. ZNF536, A Novel Zinc Finger Protein Specifically Expressed In The Brain, Negatively Regulates Neuron Differentiation Via Repressing RA-Induced Gene Transcription. Mol. Cell. Biol. 29:3633-43, 2009. doi: 10.1128/MCB.00362-09.
66. Zhili Rong, Andrew Wang, Zhiyong Li, Yongming Ren, Long Cheng, Yinghua Li, Yinyin Wang, Fangli Ren, Xiaoning Zhang, Jim Hu and Zhijie Chang*. IL-17RD (Sef or IL-17RLM) Interacts with IL-17 Receptor and Mediates IL-17 Signaling. Cell Research. 19:208-215, 2009. doi: 10.1038/cr.2008.320.
67. Fang Wang, Haiwei Zhang, Xinjun Zhang, Yinyin Wang, Fangli Ren, Xiaoning Zhang, Yonggong Zhai*, and Zhijie Chang*. Varp interacts with Rab38 and functions as its potential effector. Bioch. Bioph. Res. Com. 372:162-167, 2008. doi: 10.1016/j.bbrc.2008.05.017.
68. Xueni Li, Mei Huang, Huiling Zheng, Yinyin Wang, Fangli Ren, Yu Shang, Yonggong Zhai, David M. Irwin, Yuguang Shi, Di Chen, and Zhijie Chang*. CHIP promotes Runx2 degradation and negatively regulates osteoblast differentiation. Journal of Cell Biology. 181(6):959-972, 2008. doi: 10.1083/jcb.200711044.
69. Yongming Ren, Long Cheng, Zhili Rong, Zhiyong Li, Yinghua Li, Xinjun Zhang, Shiqin Xiong, Jim Hu, Xin-Yuan Fu, Zhijie Chang*. hSef potentiates EGF-mediated MAPK signaling through affecting EGFR trafficking and degradation. Cellular Signalling. 20:518-533, 2008. doi: 10.1016/j.cellsig.2007.11.010.
70. REN Yong-Ming, RONG Zhi-Li, Li Zhi-Yong, CHENG Long, LI Ying-Hua, WANG Yin-Yin, REN Fang-Li, David M. Irwin, CHANG Zhijie*. c-Cbl mediated ubiquitination and degradation of hSef. Pro. Biochem. Biophys. 35(1), 43-49, 2008.
71. Ren-Feng Li, Yu Shang, Di Liu, Ze-Song Ren, Zhijie Chang*, Sen-Fang Sui*. Differential Ubiquitination of Smad1 Mediated by CHIP: Implications in the Regulation of the Bone Morphogenetic Protein Signaling Pathway. Journal of Molecular Biology. 374:777-790, 2007. doi: 10.1016/j.jmb.2007.09.082. (co-corresponding authors).
72. Zhili Rong, Long Cheng, Yongming Ren, Zhiyong Li, Yinghua Li, Xueni Li, Hongge Li, Xin-Yuan Fu, Zhijie Chang*. Interleukin-17F Signaling Requires Ubiquitination of Interleukin-17 Receptor via TRAF6. Cellular Signalling. 19:1514-1520, 2007. doi: 10.1016/j.cellsig.2007.01.025.
73. Yongming Ren, Zhiyong Li, Zhili Rong, Long Cheng, Yinghua Li, Zhen Wang, Zhijie Chang*. Tyrosine 330 in hSef is critical for the localization and the inhibitory effect on FGF signaling. Bioch. Bioph. Res. Com. 354: 741-746, 2007. doi: 10.1016/j.bbrc.2007.01.037.
74. Zhijie Chang, Lorne A Babiuk, Jim Hu*. Therapeutic and Prophylactic Potential of siRNAs against SARS: Progress to Date. BioDrugs. (Invited review). 21: 9-15, 2007. doi: 10.2165/00063030-200721010-00002.
75. Yongming Ren, Long Cheng, Zhili Rong, Zhiyong Li, Yinghua Li, Hongge Li, Zhen Wang, Zhijie Chang*. hSef co-localizes and interacts with Ras in the inhibition of Ras/MAPK signaling pathway. Bioch. Bioph. Res. Com. 347: 988-993, 2006. doi: 10.1016/j.bbrc.2006.06.193.
76. Zhili Rong, Yongming Ren, Long Cheng, Zhiyong Li, Yinghua Li, Yang Sun, Hongge Li, Shiqin Xiong, and Zhijie Chang*. Sef-S, an alternative splice isoform of sef gene, inhibits NIH3T3 cell proliferation via a mitogen-activated protein kinases p42 and p44 (ERK1/2)-independent mechanism. Cellular Signalling. 19: 93-102, 2007. doi: 10.1016/j.cellsig.2006.05.033.
77. Yu Rong, Long Cheng, Hongxiu Ning, Jizhen Zou, Yuanjiang Zhang, Fang Xu, Li Liu, Zhijie Chang*, and Xin-Yuan Fu*. WT1 and STAT3 synergistically promote cell proliferation: a possible mechanism in sporadic Wilms' tumor. Cancer Research. 66: 8049-8057, 2006. (co-corresponding authors) doi: 10.1158/0008-5472.CAN-06-1172.
78. Xinjun Zhang, Xi He, Xin-Yuan Fu*, Zhijie Chang*. Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics. Journal of Cell Science. 119: 1053-62, 2006. (co-corresponding authors) doi: 10.1242/jcs.02810.
79. Zhijie Chang* and Jim Hu*. RNAi therapeutics: Can siRNAs conquer SARS? News and Commentary (invited). Gene Therapy. 13: 871-872, 2006. (co-corresponding authors) doi: 10.1038/sj.gt.3302682.
80. Yue Chen, Ying-Hua Li, Xi-Ping Chen, Li-Min Gong, Shu-Ping Zhang, Zhijie Chang, Xiu-Fang Zhang*, Xin-Yuan Fu*, Li Liu*. Point mutation at single tyrosine residue of novel oncogene NOK abrogates tumorigenesis in nude mice. Cancer Research. 65: 10838-10846, 2005. doi: 10.1158/0008-5472.CAN-05-1091.
81. Hong Xin, Xialian Xu, Linyu Li, Hongxiu Ning, Yu Rong, Yu Shang, Yinyin Wang, Xin-Yuan Fu, and Zhijie Chang*. CHIP controls the sensitivity of TGF-b signaling by maintaining the basal level of Smad3 through ubiquitin-mediated degradation. J. Biol. Chem. 280: 20842-20850, 2005. doi: 10.1074/jbc.M412275200.
82. Tieshi Li, Yuanjiang Zhang, Ling Fu, Changming Yu, Xueni Li, Yinghua Li, Xinjun Zhang, Zhili Rong, Yinyin Wang, Hongxiu Ning, Rong Liang, Wei Chen, Lorne A. Babiuk, and Zhijie Chang*. siRNA targeting Leader sequence of SARS-CoV inhibited the virus replication. Gene Therapy. 12: 751-761, 2005. doi: 10.1038/sj.gt.3302479.
83. Ning Hong-Xiu, Chen Yue, Zhang Yuan-Jiang, Rong Yu, Wu Xiao-Jun, Zhang Xiu-Fang, and Chang Zhi-Jie*. A LIF mutation at the 29th amino acid totally abolished the biological functions. Pro. Biochem. Biophys. 32: 318-324, 2005.
84. Li Liu, Xin-Zi Yu, Tie-Shi Li, Lian-Xia Song, Pei-La Chen, Ta-Lin Suo, Ying-Hua Li, Shi-Dong Wang, Yue Chen, Yong-Ming Ren, Shu-Ping Zhang, Zhi-Jie Chang, Xin-Yuan Fu*. A novel protein tyrosine kinase NOK that shares homology with platelet-derived growth factor/fibroblast growth factor receptors induces tumorigenesis and metastasis in nude mice. Cancer Research. 64: 3491-3499，2004. doi: 10.1158/0008-5472.CAN-03-2106.
85. Keiko Tamai, Xin Zeng, Chunming Liu, Xinjun Zhang, Yuko Harada, Zhijie Chang, and Xi He*. A mechanism for Wnt co-receptor activation. Molecular Cell. 13: 149-156, 2004. doi: 10.1016/s1097-2765(03)00484-2.
86. Linyu Li, Hong Xin, Xialian Xu, Mei Huang, Xinjun Zhang, Yue Chen, Shuping Zhang, Xin-Yuan Fu* and Zhijie Chang*. CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription. Mol. Cell. Biol. 24: 856-864, 2004. (co-corresponding authors). doi: 10.1128/mcb.24.2.856-864.2004.
87. Shiqin Xiong, Qiuhui Zhao, Zhili Rong, Guanrong Huang, Yiling Huang, Peila Chen, Shuping Zhang, Li Liu, and Zhijie Chang*. hSef inhibits PC12 cell differentiation by interfering with Ras-Mitogen-activated MAPK signaling. J. Biol. Chem. 278: 50273-50282, 2003. doi: 10.1074/jbc.M306936200.
88. Ye Xiongjun, Zhang Zhiwen, Zhang Xinjun, Lin Guiting, Xiong Shiqin, Jin Guihua, Han Liang, Huang Shisi, Chen Peila, Ai Junkui, Xin Dianqi, Guo Yinglu, and Chang Zhijie*. Functional interaction of TCF4 with ATF5 to regulate the Wnt signaling pathway. Chinese Science Bulletin. 48: 668-670, 2003. doi: 10.1007/BF03325652
Book(chapter)s:
1. Haimeng Zhou, Zengyi Chang, J Fang, Anming Meng, Zhijie Chang, Bingbin Yu. Translated from Lehninger Principles of Biochemistry. (4 chapters). Original authors: David L. Nelson, Michael M. Cox. High Education Press. 2005. In Chinese (Chapters 12, 13, 19 and 23).
2. Chief Editor, Libo Yao, Associate Editor, Zhijie Chang. Authors, Wenli Ma, Jicun Wang, Yingli Wang, Zonghou Shen, Zuohua Feng, Liguang Sun, Xinping Liu, Qingjie Li, Xiao Yang, Juhui Wu, Fengtian He, Hong Zhou, Libo Yao, Xiaohong Qian, Zhuzhong Mei, Zhijie, Chang Xianlu Zeng, Binghua Jiao. Molecular Biology Experiments For Medical Graduate Students. People’s Health Press. 2002. In Chinese (Chapter 18 and organization).
3. Jinyuan Liu, Zhijie Chang, Guangrong Zhao, Ji Li, Yaoting Wu. A Protocol for Molecular Biology for Graduate Students. Tsinghua University Press. Nov. 2002. In Chinese (Chapters 6 and 12).
4. Qingyu We, Jinyuan Liu, Zhijie Chang, Liping Xie, Mingxing Duan, Jinchun Chen. An Experimental Guide for Introduction of Modern Biology. Tsinghua University Press. Dec. 2002. In Chinese (Chapters 5 and 6).
Patents:
1. Zhijie Chang, Fangli Ren, Yinyin Wang, Yuting Lin. Method, application, and reagent kit for determining the surgical margin of cancer; Patent application number: 202210584510.2.
2. Zhijie Chang, Fangli Ren, Yinyin Wang, Yuting Lin, Wang Ying. Method, application, and drug for preventing cancer recurrence, inhibiting or reversing normal tissue inflammation and carcinogenesis. Patent application number: 202210584526.3.
3. Zhijie Chang, Fangli Ren, Yinyin Wang, Mengdi Li. Treatment method and application of cancer caused by activation of MAPK/ERK pathway and CREPT-CDK9 complex. Patent application number: 202210584703.8.
4. Zhijie Chang, Fangli Ren, Yinyin Wang, Danhui Ma, Ye Tian. The use of CREPT in the treatment of prostate cancer. Patent application number: 202210770054.0.
5. Zhijie Chang, Fangli Ren, Yinyin Wang, Danhui Ma, Ye Tian. The Use of CREPT in staging, monitoring progress, and/or judging prognosis of prostate cancer. Patent application number: 202210621952.
6. Shiqin Xiong, Zhijie Chang, Xin-Yuan Fu. Cloning and application of IL-17R like molecular. Filed date: 06/30/2003, Application No. 10/608,449. Issued date: Nov. 28, 2006. American Patent No.: US7141390B2.
7. Shiqin Xiong, Zhijie Chang, Xin-Yuan Fu. Gene and application of human IL-17R like molecular. Issued date: Aug. 31, 2005. Chinese Patent No.: ZL02123447.7. Chinese Certificate No.: 252260.
8. Shiqin Xiong, Zhijie Chang, Xin-Yuan Fu. Gene and application of another human IL-17R like molecular. Issued date: Jul. 20, 2005. Chinese Patent No.: ZL02123540.6. Chinese Certificate No.: 218350.
9. Shiqin Xiong, Zhijie Chang, Xin-Yuan Fu. Gene and application of mouse IL-17R like molecular. Issued date: Jul. 20, 2005. Chinese Patent No.: ZL02123542.2. Chinese Certificate No.: 218351.
10. Shiqin Xiong, Zhijie Chang, Xin-Yuan Fu. Gene and application of another mouse IL-17R like molecular. Issued date: Jun. 8, 2005. Chinese Patent No.: ZL02123541.4. Chinese Certificate No.: 212161.
11. Shiqin Xiong, Zhijie Chang, Xin-Yuan Fu. Application of a cell line overexpressing human IL-17R like molecular. Issued date: Aug. 31, 2005. Chinese Patent No.: ZL02123965.7. Chinese Certificate No.: 225400.
12. Li Liu, Xin -Yuan Fu, Zhijie Chang, Tieshi Li, Yinghua Li, Liangxia Song, Xinzi Yu, Peila Chen. Cloning and application of human NOK, a novel oncogene with kinase-domain promoting tumorigenesis and metastasis. Issued date: Mar. 22, 2006. Chinese Patent No.: ZL03105109.X. Chinese Certificate No.: 255182.
13. Li Liu, Xin-Yuan Fu, Zhijie Chang, Tieshi Li, Yinghua Li, Liangxia Song, Xinzi Yu, Peila Chen. Cloning and application of mouse NOK, a novel oncogene with kinase-domain promoting tumorigenesis and metastasis. Issued date: Mar. 22, 2006. Chinese Patent No.: ZL03105110.3. Chinese Certificate No.: 255183.
14. Zhijie Chang, Yu Rong, Hongxiu Ning, Chunhua Wang, Yinyin Wang, Yonggong Zhai. Method and application of a cell model for drug screening. Issued date: Oct. 18, 2006. Chinese Patent No.: ZL03105109.X. Chinese Certificate No.: 255182.
15. Li Liu, Xin-Yuan Fu, Zhijie Chang, Shuping Zhang. Establishment and application of a stable cell line expressing human NOK, a novel oncogene with kinase-domain promoting tumorigenesis and metastasis. Issued date: Sept. 13, 2006. Chinese Patent No.: ZL200410037888.2. Chinese Certificate No.: 283325.
16. Li Liu, Xin-Yuan Fu, Zhijie Chang, Shuping Zhang. Cloning and application of a chimeric protein with the extracellular domain of mouse EPOR and the intracellular domain of human NOK. Issued date: Sept. 13, 2006. Chinese Patent No.: ZL200410037889.7. Chinese Certificate No.: 283336.
